Coding

Part:BBa_K945006:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-27)


Pichia pastoris optimized His-CrtE-PTS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1346
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1346
    Illegal AgeI site found at 1037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301


Design Notes

The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.


Source

This sequence is originally derived from the CDS for geranylgeranyl pyrophosphate synthase from Pantoea ananatis.

References